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After cutting a window in the side of the spathe (the green outer "leaf" of the bloom), Chad Miller applied pollen, by hand. A highly-specialized piece of equipment was used to cut into the spathe: a serrated blade from a $5 Halloween pumpkin carving kit!

After cutting a window in the side of the spathe (the green outer "leaf" of the bloom), Chad Miller applied pollen, by hand. A highly-specialized piece of equipment was used to cut into the spathe: a serrated blade from a $5 Halloween pumpkin carving kit. | Download this photo.

K-State corpse flower blooms again, less than a year later

Rare repeat offers better preparation, research opportunities

May 30, 2018

MANHATTAN, Kan. — For the second consecutive year, a titan arum (better known as a corpse flower) has bloomed at Kansas State University.

Last year’s bloom was the first for the tropical plant, a gift to the university back in 2001. In its native southeast Asian habitat, titan arum can take more than 10 years to produce its first bloom.

“It was a little surprising to see another one so quick,” said Chad Miller, an associate professor of landscape horticulture at K-State. “But it sort of makes sense – we have a unique rare plant in the sense that it has several leaves, in addition to an inflorescence at the same time. Normally a plant has one leaf or one inflorescence at a time. Those leaves are basically helping to build the tuber down under the soil, so that it has enough carbohydrates or nutrition down there to support the energy needed to develop a large inflorescence, and the accompanying smell. And because we didn’t pollinate and have seed set last year, more energy was able to be stored.”

While Miller was caught by surprise in 2017, this year he knew what to look for. He said he noticed the developing inflorescence about three weeks ago.

“Because we were finishing the semester, I had two finals to give and projects to grade, so I had to make sure all that was done first,” he said. 

While the titan arum is a rare plant, native only to Sumatra and Java, modern greenhouse technology has allowed it to be cultivated around the globe. A list of just some of the other places it can be found in the United States includes California, Arizona, North Carolina, New York and Illinois.

In its native habitat, the titan arum is pollinated by flies and carrion beetles that are attracted to the plant by its strong (even overpowering) stench. In greenhouses and botanical gardens, the plant is pollinated by overnight delivery.

“The folks at the Chicago Botanic Garden were very gracious to share some pollen, several vials of pollen that had been harvested at different times and stages of the blooming process of their recent inflorescence,” Miller said.

Using a small paintbrush, Miller carefully applied the donated pollen to the lower part or female florets of the K-State bloom. If there was successful fertilization, he should be able to harvest seed in about 6 months.

“I also harvested pollen from our plant. If we can set up a long-term storage solution, I can save the pollen and then certainly share it with Chicago, and other gardens and institutions when they have a flowering event or two, that they might want to do the cross pollination,” he said.

The educational displays, the live feed, talking with visitors – it all makes for a very non-typical day at the office, or greenhouse.

“I’ve told visitors this is really – for lack of better words – it's a ‘side show’ to what I normally do. I’m still learning about this plant, as everybody else is,” Miller said.

“I look forward to more opportunities to learn more about it.”

Chad Miller (right), discusses the titan arum or "corpse flower" bloom at Kansas State University on Saturday, May 19, 2018.

Chad Miller (right), discusses the titan arum or "corpse flower" bloom at Kansas State University on Saturday, May 19, 2018. | Download this photo.

 

Sampling the gases emitted by the bloom. Chemical compounds will be analyzed.

Sampling the gases emitted by the bloom. Chemical compounds will be analyzed. | Download this photo.

 

Looking down inside the bloom.

Looking down inside the bloom. | Download this photo.

 

In the jungles of Sumatra and Java, titan arum is pollinated by flies and beetles; in greenhouses and botanical gardens, titan arum is pollinated by overnight delivery. The pollen was shared by the Chicago Botanic Garden. A small artist's paint brush was used to apply the pollen.

In the jungles of Sumatra and Java, titan arum is pollinated by flies and beetles; in greenhouses and botanical gardens, titan arum is pollinated by overnight delivery. The pollen was shared by the Chicago Botanic Garden. A small artist's paint brush was used to apply the pollen. | Download this photo.

 

The peduncle is inside the spathe. "Male" pollen is towards the top, the cluster of yellow. The "female" flowers are below.

The peduncle is inside the spathe. "Male" pollen is towards the top, the cluster of yellow. The "female" flowers are below. | Download this photo.

 

Using the paintbrush, bits of pollen are dabbed onto each of the female flowers. With any luck, the flowers will grow small round "fruit." The pollen will be harvested, put into long-term storage, and shared with other universities and gardens.

Using the paintbrush, bits of pollen are dabbed onto each of the female flowers. With any luck, the flowers will grow small round "fruit." The pollen will be harvested, put into long-term storage, and shared with other universities and gardens. | Download this photo.

 

SIDEBAR: 

The smell of death around you

If you’ve never been in the vicinity of a titan arum or “corpse flower” in full bloom, and if you’ve never encountered a decomposing human body, the following words might help.

Imagine a big bag of fruits and vegetables from your favorite farmers market, forgotten in the back of the car. For two weeks. In August.

That’s a pretty good idea of the wallop titan arum delivers to your olfactories.

“I was here when the bloom peaked, late Friday night,” said Kathleen Sellens, a K-State graduate student who recently completed her PhD in analytical chemistry. “It was – ugh, I was full-on gagging!”

But it was exactly where Sellens wanted and needed to be. Sellens and her assistants – graduate student Jay Sibbitts, and undergraduates Danica Smith and Abigail McCormick – spent much of Friday and most of Saturday – at least 22 hours – collecting data on the inflorescence. A infrared meter was used to measure the temperature of the bloom, every hour. The highest temperature reading, 90°F, was noted at the very top of the spadix at 11:30 Friday night. The spadix is tall, pale yellow protrusion sticking up from the center of the bloom.

After the temperature reading, a pump was used to collect air samples of the stench.

“We’re using a vacuum pump to pull air through a solid base extraction tube, gently draped over the edge of the bloom,” Sellens said. “The end of the tube is fitted with a special cartridge; it looks like a small foam filter. The vacuum pulls the air from inside the bloom, including all of the volatile compounds that are being released. The compounds are trapped in the cartridge, which is changed out at carefully timed intervals.”

“Back in the lab, we’ll be extracting them with hexane, and then we’re using a gas chromatograph and mass spectrometer to test and determine the molecular weight of the compounds.”

By late Tuesday, Sellens had some initial findings.

“Today we were able to discover multiple compounds that changed in concentration or presence over the course of the bloom,” she said. “We will work on identifying their chemical names the rest of the week.”

“The few that we have identified include sulfur and amine compounds which were expected due to the smell.”

 Sellens hopes to make an important contribution to a very small pool of data.

“This has only been tested in maybe one or two other places,” she said. “Cornell has a research paper they’ve released on this type of plant, but other than that, very little research has been done on it.”

At a glance

Less than a year after producing its first bloom, K-State’s titan arum has done it again, offering researchers another opportunity to research the rare plant.

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Notable quote

“I’ve told visitors this is really – for lack of better words – it's a ‘side show’ to what I normally do. I’m still learning about this plant, as everybody else is.”

— Chad Miller, associate professor of landscape horticulture, Kansas State University

Source

Chad Miller
ctmiller@ksu.edu
785-532-1416

Written by

Randall Kowalik
rkowalik@ksu.edu
785-532–0994

 

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K‑State Research and Extension is a short name for the Kansas State University Agricultural Experiment Station and Cooperative Extension Service, a program designed to generate and distribute useful knowledge for the wellbeing of Kansans.
Supported by county, state, federal and private funds, the program has county extension offices, experiment fields, area extension offices and regional research centers statewide. Its headquarters is on the K‑State campus in Manhattan.